4YZF
Crystal structure of the anion exchanger domain of human erythrocyte Band 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.825, 171.957, 271.700 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 37.718 - 3.500 |
| R-factor | 0.2752 |
| Rwork | 0.274 |
| R-free | 0.29010 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.369 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.780 | |
| Number of reflections | 84446 | |
| <I/σ(I)> | 18 | 1.6 |
| Completeness [%] | 97.3 | 96.5 |
| Redundancy | 5.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100 mM Tris-HCl (pH 8.0), 22-26 %(v/v) PEG300, 250 mM CH3COOK |
