4YYU
Ficin C crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.901 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.939, 88.939, 56.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.764 - 1.177 |
| R-factor | 0.1403 |
| Rwork | 0.139 |
| R-free | 0.15660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yyr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.407 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.764 | 1.210 |
| High resolution limit [Å] | 1.177 | 1.180 |
| Rmerge | 0.089 | 0.327 |
| Number of reflections | 81464 | |
| <I/σ(I)> | 9.9 | 3.5 |
| Completeness [%] | 96.4 | 93.6 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG400, 0.1 M HEPES pH, 0.2 M MgCl2 |
