4YXP
The structure of the folded domain of the signature multifunctional protein ICP27 from herpes simplex virus-1 reveals an intertwined dimer.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.330, 133.620, 44.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.553 - 1.920 |
R-factor | 0.1699 |
Rwork | 0.168 |
R-free | 0.20260 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.798 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1977) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.553 | 1.989 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.061 | 0.640 |
Number of reflections | 46524 | |
<I/σ(I)> | 19.02 | 2.86 |
Completeness [%] | 100.0 | 99 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | Crystals grown from reservoir solutions consisting of 0.09 M (NaF, NaBr, NaI), 0.1 M (Imidazole, MES) buffer system pH 6.5, 30% (Ethylene glycol, PEG 8K) [Morpheus HT96 B2 Molecular Dimensions] and 0.12 M (1,6-Hexanediol, 1-Butanol 1,2-Propanediol (racemic), 2-Propanol, 1,4-Butanediol, 1,3-Propanediol), 0.1 M (Imidazole, MES) buffer system pH 6.5, 30% (Ethylene glycol, PEG 8K) [Morpheus HT96 D2 Molecular Dimensions] |