4YVW

crystal structure of an enterovirus 71/coxsackievirus A16 chimeric virus-like particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2013-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.281579
Spacegroup name	P 42 3 2
Unit cell lengths	349.151, 349.151, 349.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.960 - 3.800
R-factor	0.2876
Rwork	0.286
R-free	0.32430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pov
RMSD bond length	0.005
RMSD bond angle	1.123
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.870
High resolution limit [Å]	3.800	10.290	3.800
Rmerge	0.177	0.056
Rmeas	0.204	0.068
Rpim	0.130	0.038
Total number of observations	123302
Number of reflections	56842
<I/σ(I)>	4.1
Completeness [%]	79.2	71.6	74.4
Redundancy	2.2	2.6	1.8
CC(1/2)		0.992	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	0.1M HEPES, 0.5M Magnesium formate