4YVW
crystal structure of an enterovirus 71/coxsackievirus A16 chimeric virus-like particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.281579 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 349.151, 349.151, 349.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.960 - 3.800 |
| R-factor | 0.2876 |
| Rwork | 0.286 |
| R-free | 0.32430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pov |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 10.290 | 3.800 |
| Rmerge | 0.177 | 0.056 | |
| Rmeas | 0.204 | 0.068 | |
| Rpim | 0.130 | 0.038 | |
| Total number of observations | 123302 | ||
| Number of reflections | 56842 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 79.2 | 71.6 | 74.4 |
| Redundancy | 2.2 | 2.6 | 1.8 |
| CC(1/2) | 0.992 | 0.374 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, 0.5M Magnesium formate |
