4YVS

crystal structure of the virus-like particle of a c4 strain EV71

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2012-10-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97853
Spacegroup name	P 42 3 2
Unit cell lengths	349.746, 349.746, 349.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.964 - 3.650
R-factor	0.2721
Rwork	0.271
R-free	0.30030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pov
RMSD bond length	0.005
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.780
High resolution limit [Å]	3.650	7.850	3.650
Rmerge	0.150	0.047
Rmeas	0.163	0.054
Rpim	0.101	0.025	0.741
Total number of observations	227650
Number of reflections	72397
<I/σ(I)>	5.7
Completeness [%]	89.2	84.7	84
Redundancy	3.1	3.9	2.5
CC(1/2)		0.997	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	290	0.1M Imidazole, 1M sodium acetate