4YUE

Mouse IL-2 Bound to S4B6 Fab Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	.97945
Spacegroup name	P 21 21 21
Unit cell lengths	43.080, 77.560, 169.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.146 - 2.194
R-factor	0.2046
Rwork	0.203
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5i
RMSD bond length	0.007
RMSD bond angle	1.198
Data reduction software	XDS (20140110)
Data scaling software	XDS (20140110)
Phasing software	PHASER
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.200		2.330
High resolution limit [Å]	2.190	6.560	2.190
Rmerge	0.107	0.049	1.306
Rmeas	0.116	0.054	1.421
Total number of observations	192408
Number of reflections	29462	1229	4609
<I/σ(I)>	12.11	35.16	1.47
Completeness [%]	98.5	98.1	97
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	295	0.1 M Bis-Tris propane, pH 6.5, 0.2 M sodium fluoride, 20% PEG3350