4YTX

Crystal structure of Ups1-Mdm35 complex with PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	90
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	208.642, 154.670, 99.012
Unit cell angles	90.00, 104.42, 90.00

Refinement procedure

Resolution	43.230 - 3.200
R-factor	0.251
Rwork	0.251
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ytw
RMSD bond length	0.014
RMSD bond angle	1.700
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.123
Number of reflections	50582
<I/σ(I)>	19.4	2.5
Completeness [%]	100.0	100
Redundancy	7.7	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 10% PEG 6000, 5% MPD