4YPB
Precleavage 70S structure of the P. vulgaris HigB DeltaH92 toxin bound to the AAA codon
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 214.112, 453.875, 607.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.932 - 3.400 |
R-factor | 0.2035 |
Rwork | 0.202 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3I9B and 3I9C |
RMSD bond length | 0.005 |
RMSD bond angle | 0.836 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.500 | |
High resolution limit [Å] | 3.400 | 35.000 | 3.400 |
Rmerge | 0.213 | 0.014 | 1.047 |
Rmeas | 0.234 | 0.017 | 1.148 |
Total number of observations | 4745905 | ||
Number of reflections | 794344 | 671 | 66224 |
<I/σ(I)> | 8.24 | 45.1 | 1.75 |
Completeness [%] | 98.9 | 76.7 | 99.6 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN |