4YON
P-Rex1:Rac1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.510, 111.250, 158.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.560 - 1.950 |
| R-factor | 0.1633 |
| Rwork | 0.162 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2DFK 3sbd |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.794 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.560 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.207 | 1.337 |
| Rpim | 0.063 | 0.404 |
| Number of reflections | 60929 | |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 sodium MES, pH 6.5, 0.2M sodium acetate, 15% (w/v) PEG 8000 |
