4YOC

Crystal Structure of human DNMT1 and USP7/HAUSP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	110.231, 111.619, 163.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.419 - 2.916
R-factor	0.21
Rwork	0.208
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3swr 2ylm
RMSD bond length	0.003
RMSD bond angle	0.892
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.146	0.053	0.858
Total number of observations	615406
Number of reflections	44471
<I/σ(I)>	8.1
Completeness [%]	100.0	99.8	100
Redundancy	13.8	14	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	6-8% PEG 3350, 200 mM potassium acetate