4YOB
Crystal Structure of Apo HIV-1 Protease MDR769 L33F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-05-08 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.978 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 45.754, 45.754, 102.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.328 - 1.504 |
R-factor | 0.1769 |
Rwork | 0.176 |
R-free | 0.19430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.984 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 102.220 |
High resolution limit [Å] | 1.500 |
Number of reflections | 17812 |
<I/σ(I)> | 34.4 |
Completeness [%] | 99.1 |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 2.4M ammonium sulfate, 0.1 M MES, pH 6.2; 2.4 M ammonium sulfate, 0.1 M HEPES, pH 6.8 |