4YNY

Crystal structure of monoclonal anti-human podoplanin antibody NZ-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-14
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	42.883, 57.157, 101.520
Unit cell angles	97.92, 101.53, 105.37

Refinement procedure

Resolution	31.486 - 1.584
R-factor	0.1702
Rwork	0.168
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zan
RMSD bond length	0.017
RMSD bond angle	1.552
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.500
High resolution limit [Å]	1.584	1.584
Rmerge	0.130	0.767
Number of reflections	118170
<I/σ(I)>	24.5	2.3
Completeness [%]	97.7	89
Redundancy	6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, sodium citrate
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, sodium citrate