4YM4

Truncated Human TIFA in complex with its Thr9 phosphorylated N-terminal peptide 1-15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2014-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 42 3 2
Unit cell lengths	113.205, 113.205, 113.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.120
R-factor	0.2145
Rwork	0.213
R-free	0.23860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.210
High resolution limit [Å]	3.100	6.610	3.100
Rmerge	0.161	0.040	0.885
Rmeas	0.164	0.041	0.909
Rpim	0.035	0.009	0.206
Total number of observations	107113
Number of reflections	4922
<I/σ(I)>	6.3
Completeness [%]	100.0	100	100
Redundancy	21.8	18.8	19.2
CC(1/2)		0.999	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.1 M DL-malic acid pH7.0