4YL8

Crystal structure of the Crumbs/Moesin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.115, 65.121, 83.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 1.500
R-factor	0.1599
Rwork	0.159
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ef1
RMSD bond length	0.006
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.065	0.039	0.658
Total number of observations	398670
Number of reflections	55529
<I/σ(I)>	13.2
Completeness [%]	99.8	96.6	100
Redundancy	7.2	6.3	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% PEG3350, 0.2 M ammonium iodine