4YL8
Crystal structure of the Crumbs/Moesin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.115, 65.121, 83.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.800 - 1.500 |
R-factor | 0.1599 |
Rwork | 0.159 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ef1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.047 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.065 | 0.039 | 0.658 |
Total number of observations | 398670 | ||
Number of reflections | 55529 | ||
<I/σ(I)> | 13.2 | ||
Completeness [%] | 99.8 | 96.6 | 100 |
Redundancy | 7.2 | 6.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG3350, 0.2 M ammonium iodine |