4YKE

Crystal structure of eukaryotic Mre11 catalytic domain from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	199.4
Detector technology	CCD
Collection date	2012-11-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	56.722, 56.555, 304.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.517 - 2.783
R-factor	0.2015
Rwork	0.198
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fbq
RMSD bond length	0.003
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.780	2.780
Rmerge		0.884
Number of reflections	25153
<I/σ(I)>	11.18	1.81
Completeness [%]	98.1	89.5
Redundancy	6.7	6.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium citrate tribasic, PEG3350