4YKE
Crystal structure of eukaryotic Mre11 catalytic domain from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 199.4 |
Detector technology | CCD |
Collection date | 2012-11-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.722, 56.555, 304.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.517 - 2.783 |
R-factor | 0.2015 |
Rwork | 0.198 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fbq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.683 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.884 | |
Number of reflections | 25153 | |
<I/σ(I)> | 11.18 | 1.81 |
Completeness [%] | 98.1 | 89.5 |
Redundancy | 6.7 | 6.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Ammonium citrate tribasic, PEG3350 |