4YII

Structure of an APC2-UBCH10 complex reveals distinctive cullin-RING ligase mechanism for Anaphase-promoting complex/Cyclosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.2826
Spacegroup name	C 1 2 1
Unit cell lengths	133.113, 33.188, 51.963
Unit cell angles	90.00, 100.12, 90.00

Refinement procedure

Resolution	44.272 - 1.804
R-factor	0.1985
Rwork	0.196
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ldd 1i7k
RMSD bond length	0.013
RMSD bond angle	1.499
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.347
Number of reflections	21055
<I/σ(I)>	28.4
Completeness [%]	94.0	68.2
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	PEG 3000, MES