4YIA

Structural mechanism of hormone release in thyroxine binding globulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 2 21
Unit cell lengths	41.980, 56.070, 173.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.570 - 1.580
R-factor	0.20592
Rwork	0.205
R-free	0.22924
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.199
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	26.570
High resolution limit [Å]	1.580
Number of reflections	52812
<I/σ(I)>	3.5
Completeness [%]	98.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		290	PEG 4000