4YHC
Crystal structure of the WD40 domain of SCAP from fission yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-08 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.009, 109.714, 103.454 |
| Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.050 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22930 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.836 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 51974 |
| <I/σ(I)> | 27.3 |
| Completeness [%] | 99.1 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 20% (v/v) PEG 3350, 0.2M Na3Citrate |
