4YGO
Dodecameric structure of spermidine N-acetyltransferase from Vibrio cholerae in intermediate state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.118, 135.793, 72.848 |
Unit cell angles | 90.00, 117.13, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.18368 |
Rwork | 0.180 |
R-free | 0.25274 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3EG7 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.762 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 | 0.590 |
Number of reflections | 46610 | |
<I/σ(I)> | 25.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.01 M CaCl2, 0.1 M Tris HCl, 20% Methanol, 25 % MPD |