4YG6
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977408 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.200, 140.280, 54.600 |
Unit cell angles | 90.00, 90.78, 90.00 |
Refinement procedure
Resolution | 36.200 - 1.460 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yg3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.200 | 1.540 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.074 | 0.333 |
Number of reflections | 93062 | |
<I/σ(I)> | 11 | 4.2 |
Completeness [%] | 99.0 | 99.5 |
Redundancy | 4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M Phosphate citrate, 1.6 M Sodium dihydrogen phosphate, 0.4 M di-Potassium hydrogen phosphate. |