4YFX

Escherichia coli RNA polymerase in complex with Myxopyronin B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	P 21 21 21
Unit cell lengths	188.520, 205.177, 310.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.947 - 3.844
R-factor	0.2368
Rwork	0.236
R-free	0.29990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4IGC
RMSD bond length	0.014
RMSD bond angle	2.146
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.800
Number of reflections	106246
<I/σ(I)>	9
Completeness [%]	93.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	300	0.1M HEPES-HCL , 0.2M CaACETATE, 15% PEG400