4YFK
Escherichia coli RNA polymerase in complex with squaramide compound 8.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 186.105, 206.438, 307.993 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.965 - 3.571 |
R-factor | 0.2173 |
Rwork | 0.217 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4IGC |
RMSD bond length | 0.018 |
RMSD bond angle | 1.810 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.500 |
Number of reflections | 133199 |
<I/σ(I)> | 12.8 |
Completeness [%] | 96.8 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1M HEPES-HCL, 0.2M CaACETATE, 15% PEG400 |