4YFD
Crystal structure PTP delta Ig1-Fn2 in complex with IL-1RAcP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.497, 63.306, 169.350 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 48.974 - 3.253 |
| R-factor | 0.2574 |
| Rwork | 0.256 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd6 2yd9 4dep 3o4o 4yfe |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.438 | |
| Number of reflections | 25436 | |
| <I/σ(I)> | 10.7 | 2 |
| Completeness [%] | 97.3 | 92.5 |
| Redundancy | 4.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 15% PEG3350, 0.1 M ammonium sulfate and 0.1 M tri-sodium citrate |
