4YEF
beta1 carbohydrate binding module (CBM) of AMP-activated protein kinase (AMPK) in complex with glucosyl-beta-cyclododextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.718, 68.394, 92.211 |
| Unit cell angles | 111.72, 95.56, 90.06 |
Refinement procedure
| Resolution | 43.710 - 1.720 |
| R-factor | 0.17944 |
| Rwork | 0.178 |
| R-free | 0.21312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z0m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.605 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.710 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.043 | 0.628 |
| Number of reflections | 95126 | |
| <I/σ(I)> | 18.6 | 1.9 |
| Completeness [%] | 97.1 | 92.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 281.15 | 0.2 M lithium sulphate, 25 % w/v PEG 8000 and 0.1 M sodium acetate pH 4.5 |
