4YDL
Crystal structure of broadly and potently neutralizing antibody C38-VRC18.02 in complex with HIV-1 clade AE strain 93TH057gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.330, 213.089, 68.185 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 35.855 - 1.800 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.20740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3se9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1702) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.063 | 0.044 | 0.721 |
| Total number of observations | 607672 | ||
| Number of reflections | 175625 | ||
| <I/σ(I)> | 13.9 | 2 | |
| Completeness [%] | 99.0 | 98.3 | 98.9 |
| Redundancy | 3.5 | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 9.0% PEG 4000, 4.5% isopropanol, 0.1M HEPES, pH 7.5 |
