4YC6
CDK1/CKS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.817, 147.531, 87.297 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 75.090 - 2.600 |
| R-factor | 0.2268 |
| Rwork | 0.225 |
| R-free | 0.26700 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.855 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.090 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.094 | 2.080 |
| Number of reflections | 50462 | |
| <I/σ(I)> | 16.6 | 1.97 |
| Completeness [%] | 97.0 | 80.6 |
| Redundancy | 5.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.3 | 277 | 16-18% PEG 10 000, 0.1M imidazole pH 8.2 -8.4 |
