4YC2
Crystal structure of the stabilized inner domain of clade A/E HIV-1 gp120 from E. coli in complex with the antibody A32.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.801, 211.800, 72.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.020 |
| R-factor | 0.23034 |
| Rwork | 0.227 |
| R-free | 0.28774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TNM and 4RQH |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.163 | 0.880 |
| Number of reflections | 20655 | |
| <I/σ(I)> | 9.5 | 1.2 |
| Completeness [%] | 87.1 | 86.7 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 18-22% PEG 6000 or PEG8000 0.1 M Tris-HCl pH 8.5 |
