4YBQ
Rat GLUT5 with Fv in the outward-open form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.773, 151.541, 106.396 |
Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
Resolution | 36.491 - 3.270 |
R-factor | 0.2442 |
Rwork | 0.242 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o7q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.270 |
Number of reflections | 38017 |
<I/σ(I)> | 1.22 |
Completeness [%] | 99.9 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 33-35% PEG400, 0.12 M CaCl2, 0.1 M Tris-HCl pH8.0 |