4YB9

Crystal structure of the Bovine Fructose transporter GLUT5 in an open inward-facing conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	74.610, 112.150, 139.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.317 - 3.200
R-factor	0.2388
Rwork	0.237
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rat GLUT5 crystal structure
RMSD bond length	0.003
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1951))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.317	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	13346
<I/σ(I)>	11.2	0.97
Completeness [%]	67.0
Redundancy	10.2	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	277	PEG 300, Hepes, Lithium Sulfate, Sodium Chloride, HEGA-10