4YAI
Crystal structure of LigL in complex with NADH and GGE from Sphingobium sp. strain SYK-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.719, 72.714, 58.874 |
Unit cell angles | 90.00, 105.17, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.600 |
R-factor | 0.1404 |
Rwork | 0.140 |
R-free | 0.16110 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.122 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 67529 |
<I/σ(I)> | 29.8 |
Completeness [%] | 100.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Magnesium Chloride, 0.1 M Hepes pH 7.5, 25% PEG 3,350 |