4Y9W
Aspartic Proteinase Sapp2 Secreted from Candida Parapsilosis at 0.82 A Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.260, 57.660, 54.360 |
| Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
| Resolution | 11.370 - 0.830 |
| R-factor | 0.1173 |
| Rwork | 0.117 |
| R-free | 0.12450 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.913 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 11.368 | 11.376 | 0.850 |
| High resolution limit [Å] | 0.825 | 3.690 | 0.820 |
| Rmerge | 0.073 | 0.378 | |
| Rmeas | 0.096 | ||
| Rpim | 0.032 | 0.030 | 0.289 |
| Total number of observations | 2124055 | 10579 | 43021 |
| Number of reflections | 261700 | ||
| <I/σ(I)> | 13.2 | 26.6 | 2 |
| Completeness [%] | 92.3 | 44.9 | 65.5 |
| Redundancy | 8.1 | 7.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 400 |
