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4Y97

Crystal Structure of human Pol alpha B-subunit in complex with C-terminal domain of catalytic subunit

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]100
Detector technologyCCD
Collection date2013-11-17
DetectorADSC QUANTUM 315
Wavelength(s)0.9792
Spacegroup nameP 1 21 1
Unit cell lengths103.593, 137.135, 132.092
Unit cell angles90.00, 100.97, 90.00
Refinement procedure
Resolution47.680 - 2.510
R-factor0.218
Rwork0.218
R-free0.25700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3flo
RMSD bond length0.008
RMSD bond angle1.700
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.540
High resolution limit [Å]2.5002.500
Rmerge0.0840.396
Number of reflections116900
<I/σ(I)>13.352.05
Completeness [%]95.088.5
Redundancy2.82.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.6295The diffraction quality crystals are growing in 2-3 weeks in 100 mM ammonium acetate, 50 mM Na citrate pH 5.6, 10 - 10.4% w/v PEG 4000, 2 mM TCEP and 50 mM guanidine HCl.

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