4Y40
Structure of Vaspin mutant D305C V383C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.495, 152.340, 61.611 |
Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
Resolution | 19.690 - 2.200 |
R-factor | 0.1807 |
Rwork | 0.180 |
R-free | 0.20580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4if8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.080 |
Data reduction software | XDS (November 11, 2013) |
Data scaling software | XSCALE (November 11, 2013) |
Phasing software | REFMAC (5.7.0029) |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.690 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.145 | 1.554 |
Number of reflections | 61535 | |
<I/σ(I)> | 7.9 | 0.9 |
Completeness [%] | 99.6 | 96.3 |
Redundancy | 5.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 6 % (w/v) polyethylene glycol 6000, 0.1 M citirc acid pH 3.5 -4.5 |