4Y32
Crystal structure of C-terminal modified Tau peptide-hybrid 109B with 14-3-3sigma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99983 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.062, 70.283, 128.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.477 - 1.700 |
R-factor | 0.1838 |
Rwork | 0.181 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fl5 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.993 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0017) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.477 | 1.800 | |
High resolution limit [Å] | 1.700 | 5.000 | 1.700 |
Rmerge | 0.041 | 0.023 | 0.320 |
Rmeas | 0.044 | 0.025 | 0.348 |
Total number of observations | 408200 | ||
Number of reflections | 63066 | 2617 | 9807 |
<I/σ(I)> | 29.72 | 63.79 | 7.08 |
Completeness [%] | 99.6 | 96.2 | 99.9 |
Redundancy | 6.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 277 | 0.1 M Hepes/NaOH pH 7.1, 0.19 M CaCl2, 24% PEG 400, 5% glycerol |