4Y1P
Crystal structure of 3-isopropylmalate dehydrogenase (Saci_0600) from Sulfolobus acidocaldarius complex with 3-isopropylmalate and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-02-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.024, 136.719, 112.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.510 - 2.200 |
R-factor | 0.19306 |
Rwork | 0.190 |
R-free | 0.24961 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a05 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.438 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 35126 |
<I/σ(I)> | 17.9 |
Completeness [%] | 99.7 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium sulfate, 0.1 M Cacodylate (pH6.5), 0.2 M NaCl |