4Y1J

Lactococcus lactis yybP-ykoY Mn riboswitch A41U binding site mutant in presence of Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-10-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.769
Spacegroup name	P 1
Unit cell lengths	50.419, 62.510, 69.969
Unit cell angles	116.21, 101.83, 98.83

Refinement procedure

Resolution	47.400 - 2.240
R-factor	0.2017
Rwork	0.199
R-free	0.24150
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX ((phenix.refine: dev_1555))
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.053	0.550
Number of reflections	32334
<I/σ(I)>	17.6	2.1
Completeness [%]	90.0	95
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1-0.2 mM RNA in 10 mM Na Cacodylate pH 7, 50 mM NaCl, 10 mM MgCl2, 2.5 mM MnCl2. Crystallized in about 2 months with a mother liquor of 40 mM Na cacodylate pH 6, 15% MPD, 80 mM SrCl2, 12 mM spermine tetra-HCl at 21 C. Crystals were cryo-protected by addition of 20% PEG-400 directly before freezing.