4Y1H
Crystal structure of K33 linked tri-Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.938, 41.831, 47.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.420 - 1.400 |
| R-factor | 0.15952 |
| Rwork | 0.158 |
| R-free | 0.19438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.284 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.420 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.094 | 0.130 |
| Number of reflections | 11054 | |
| <I/σ(I)> | 18.51 | 12.82 |
| Completeness [%] | 98.1 | 97.16 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM Bis Tris propane (pH 7.5), 20mM sodium/potassium phosphate, 20% PEG3350 |
