4Y0W
YeaZ from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.969 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.030, 198.443, 161.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.220 - 2.500 |
| R-factor | 0.18187 |
| Rwork | 0.178 |
| R-free | 0.25024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.220 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.827 | |
| Number of reflections | 43728 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 4M Sodium formate |
