4Y0W

YeaZ from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.969
Spacegroup name	C 2 2 21
Unit cell lengths	78.030, 198.443, 161.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.220 - 2.500
R-factor	0.18187
Rwork	0.178
R-free	0.25024
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.354
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.220	2.640
High resolution limit [Å]	2.500	2.500
Rmerge		0.827
Number of reflections	43728
<I/σ(I)>	13.7	2
Completeness [%]	99.9	100
Redundancy	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	4M Sodium formate