4Y08
ONE MINUTE IRON LOADED HUMAN H FERRITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.140, 184.140, 184.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.760 - 1.340 |
| R-factor | 0.16304 |
| Rwork | 0.162 |
| R-free | 0.17347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.110 | 1.410 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.069 | 0.395 |
| Number of reflections | 60022 | |
| <I/σ(I)> | 28.2 | 8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 2.0 M magnesium chloride and 0.1 M BICINE at pH 9.0 |
