4XXW

Crystal structure of mouse Cadherin-23 EC1-2 and Protocadherin-15 EC1-2 splice variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	C 1 2 1
Unit cell lengths	157.746, 57.789, 155.264
Unit cell angles	90.00, 99.17, 90.00

Refinement procedure

Resolution	50.000 - 2.261
R-factor	0.18945
Rwork	0.187
R-free	0.23288
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4apx
RMSD bond length	0.018
RMSD bond angle	1.806
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.300
High resolution limit [Å]	2.261	6.130	2.261
Rmerge	0.079	0.043	0.402
Rmeas	0.094	0.051	0.494
Rpim	0.050	0.026	0.283
Total number of observations	200718
Number of reflections	62947
<I/σ(I)>	6.7		2.3
Completeness [%]	96.8	98.7	84.9
Redundancy	3.2	3.6	2.5
CC(1/2)		0.994	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M Ammonium Acetate 0.1 M HEPES, pH 7.5 25% (v/v) isopropanol