4XXK
Crystal structure of the Semet-derivative of the Bilin-binding domain of phycobilisome core-membrane linker ApcE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979160 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.980, 66.590, 81.420 |
Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
Resolution | 19.487 - 2.970 |
R-factor | 0.1817 |
Rwork | 0.176 |
R-free | 0.25210 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.279 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 3.130 |
High resolution limit [Å] | 2.970 | 2.970 |
Number of reflections | 8228 | |
<I/σ(I)> | 21.7 | 12.9 |
Completeness [%] | 99.3 | 97.4 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 285 | 16% up to 26% (w/v) PEG 3350, 0.25 M NaSCN |