4XWW
Crystal structure of RNase J complexed with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.120, 87.860, 249.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.591 - 1.700 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.21700 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.109 |
Data reduction software | XSCALE |
Phasing software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.102 | 0.081 | 0.697 |
Rmeas | 0.112 | 0.091 | 0.796 |
Total number of observations | 936565 | ||
Number of reflections | 158299 | 1474 | 9536 |
<I/σ(I)> | 10.69 | 16.42 | 1.92 |
Completeness [%] | 97.2 | 72.2 | 79.8 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 4000, MnCl2, MES |