4XWH
Crystal structure of the human N-acetyl-alpha-glucosaminidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 63 |
| Unit cell lengths | 205.088, 205.088, 78.398 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.320 |
| R-factor | 0.1669 |
| Rwork | 0.166 |
| R-free | 0.18579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vcc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.776 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.086 | 0.763 |
| Number of reflections | 81740 | |
| <I/σ(I)> | 20.7 | 1.85 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 9.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 1.0 M lithium sulfate, 0.01 M nickel chloride, 0.1 M Tris |
