4XVU

Structure of Get3 bound to the transmembrane domain of Nyv1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	79.440, 109.230, 111.350
Unit cell angles	63.05, 77.74, 70.17

Refinement procedure

Resolution	69.376 - 2.350
R-factor	0.1989
Rwork	0.197
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2woj 3pgf
RMSD bond length	0.005
RMSD bond angle	0.953
Data reduction software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.380	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	123349
<I/σ(I)>	5.9	1.8
Completeness [%]	95.0	85
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	15% PEG 3350, 25 mM succinic acid pH 7.0