4XVG
Crystal structure of Alkylhydroperoxide Reductase Subunit AhpF from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.040, 58.625, 94.941 |
| Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
| Resolution | 46.155 - 2.200 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1HYU |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.123 | 0.512 |
| Number of reflections | 28666 | |
| <I/σ(I)> | 20.7 | 4.69 |
| Completeness [%] | 97.6 | 97.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M Ammonium citrate tribasic, pH 7.0 20 % w/v Polyethylene glycol 3350 |
