4XTB
Crystal structure of the N-terminal domain of the human mitochondrial calcium uniporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 |
Unit cell lengths | 55.463, 55.463, 68.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.030 - 1.500 |
R-factor | 0.14152 |
Rwork | 0.140 |
R-free | 0.17626 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xsj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.636 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 23888 | |
<I/σ(I)> | 14.3 | 1.3 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.55 M lithium sulphate 0.1 M Bis-Tris propane (pH 8.0) |