4XT3
Structure of a viral GPCR bound to human chemokine CX3CL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-20 |
Detector | PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.900, 192.700, 94.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.920 - 3.801 |
R-factor | 0.2878 |
Rwork | 0.283 |
R-free | 0.32210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mbs 1f2l 3ona |
RMSD bond length | 0.003 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.920 | 3.937 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.275 | 0.555 |
Number of reflections | 4834 | |
<I/σ(I)> | 4.1 | 1.6 |
Completeness [%] | 85.0 | 55.5 |
Redundancy | 5.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.4 | 293 | PEG 300, HEPES, ammonium phosphate |