4XT2
Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with a tetrazole-containing antagonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.253, 84.390, 82.784 |
| Unit cell angles | 90.00, 104.39, 90.00 |
Refinement procedure
| Resolution | 41.688 - 1.698 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.345 |
| Data reduction software | HKL-2000 (v704) |
| Data scaling software | HKL-2000 (v704) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.698 | 1.700 |
| Rmerge | 0.059 | 0.562 |
| Number of reflections | 54343 | |
| <I/σ(I)> | 18.3 | 2.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 2 uL 500 uM protein against 100 mM Bis-Tris, 25% PEG3350 |
