4XSX
Crystal structure of CBR 703 bound to Escherichia coli RNA polymerase holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 187.025, 206.914, 310.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.708 |
R-factor | 0.2323 |
Rwork | 0.230 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ljz |
RMSD bond length | 0.005 |
RMSD bond angle | 1.015 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.830 |
High resolution limit [Å] | 3.700 | 7.950 | 3.700 |
Rmerge | 0.144 | 0.054 | |
Total number of observations | 1671904 | ||
Number of reflections | 126483 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.9 | 99.9 | 99 |
Redundancy | 13.2 | 12.2 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | MES, calcium acetate, PEG 400 |