4XRU
Structure of Pnkp1/Rnl/Hen1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.483, 187.245, 111.999 |
Unit cell angles | 90.00, 106.04, 90.00 |
Refinement procedure
Resolution | 42.930 - 3.410 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.23300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.400 |
Number of reflections | 65456 |
<I/σ(I)> | 13.9 |
Completeness [%] | 99.8 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | PEG 6000 |